![]() The identification of genes with increased expression in this phase of the life cycle of M. This indicated that nutrient uptake can be an important signal to trigger fruiting in this fungus. Expression of the glucose transporter gene increased in mycelium after the stress, coinciding with a decrease of adenylate cyclase gene transcription. The number of transcripts of the gene for plerototolysin B increased in reddish-pink mycelium and indicated an activation of the initial basidiomata production even at this culturing stage. One aegerolysin gene had a peak of expression in mycelium with primordia and a second in basidiomata, confirming their distinctiveness. In addition, 12 genes with more detailed expression profiles were analyzed by RT-qPCR. In the down-regulated group relevant genes clustered coding calmodulin, lanosterol 14 alpha demethylase and PIM1. Hydrophobin coding, glucose transporter, Rho-GEF, Rheb, extensin precursor and cytochrome p450 monooxygenase genes were grouped among the up-regulated. This analysis showed two groups of up and down-regulated genes in primordial phases of mycelia. A macroarray analysis was performed with 192 selected clones and hybridized with two distinct RNA pools extracted from mycelium in different phases of basidiomata formation. To analyze the genes involved in basidiomata development, the expression of some selected EST genes from a non-normalized cDNA library, representative of the fruiting stage of M. ![]() The morphological analysis also allowed conclusions on morphogenetic aspects. The stages of hyphal nodules, aggregation, initial primordium and differentiated primordium were detected. Changes and appearance of hyphae forming a surface layer by fusion were correlated with primordia emergence. The morphological changes in the mycelium before fructification show a pattern similar to other members of the order Agaricales. Mycelium was morphologically analyzed prior to emergence of basidiomata by stereomicroscopy, light microscopy and scanning electron microscopy. A deeper understanding of the mechanisms underlying the sexual phase of this fungus may help develop chemical, biological or genetic strategies to control the disease. ![]() This structure generates millions of basidiospores that reinfect young tissues of the same or other plants. ![]() The pathogen life cycle ends with the production of basidiocarps in dead tissues of the infected host. The hemibiotrophic fungus Moniliophthora perniciosa is the causal agent of Witches' broom, a disease of Theobroma cacao. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |